Single Cell RNA Sequencing in Plants

RNA sequencing has historically been performed at the whole tissue or organ level, a high level view that fails to capture the true heterogeneity within tissues caused by the various cell types within the organ or tissue. Single cell resolution has been widely adopted in human and mammalian systems in recent years but has failed to make the leap to plants systems due to inherent biological differences in cell types— mainly the presence of plant cell walls complicating cell preparations. The goal of this pilot is to establish single cell RNA-sequencing methods in various plant species. By establishing a method to access the single cell level resolution we can help to better understand plant systems at a finer resolution and begin to investigate the roles various cells play in our current big picture understanding.